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ATCC
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Thermo Fisher
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Corning Life Sciences
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Verlag GmbH
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Becton Dickinson
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Becton Dickinson
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Becton Dickinson
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Discovery Labware Inc
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Becton Dickinson
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Becton Dickinson
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Image Search Results
Journal: PLoS ONE
Article Title: Met Receptor Tyrosine Kinase Signaling Induces Secretion of the Angiogenic Chemokine Interleukin-8/CXCL8 in Pancreatic Cancer
doi: 10.1371/journal.pone.0040420
Figure Lengend Snippet: Serum-depleted, confluent NT or MetKD BxPC-3 (A) or ASPC-1 (B) cells were wounded with a scratch, six regions marked, and immediately imaged (0 hr) prior to treatment with HGF. Identical regions along the scratch were imaged after 3, 9, 18, and 24 hr and the values were normalized to the initial size of the scratch at 0 hr. Cell migration is reported as the % gap closure at each time point (***p<0.001; ANOVA, n = 3). MetKD reduces BxPC-3 cell invasion in response to HGF relative to control cells (C). Values represent the fold change in the number of cells/field and are reported as the mean +/− SEM (***p<0.001; ANOVA, n = 3). Live cell imaging of ASPC-1 MetKD cells plated on collagen I-coated glass bottom cell culture dishes show decreased cell migration in response to 100 ng/mL HGF (D). Images were collected every 2 min for a total of 120 min and data is expressed as the mean path length +/− SEM per condition (***p<0.001; ANOVA, n>30 cells).
Article Snippet: For live cell migration on collagen I, cells were serum-depleted overnight (1% FBS) and then plated onto 35 mm glass bottom tissue culture plates pre-coated with 15 µg/mL
Techniques: Migration, Live Cell Imaging, Cell Culture